Abstract
[(125)I]RTI-55-membrane binding assay-guided fractionation and separation of a water-soluble extract of the roots of Polygala tenuifolia gave five new oligosaccharide derivatives, polygalatenosides A-E (1-5). The structures of these new oligosaccharides were established on the basis of spectroscopic evidence. Polygalatenosides A and B (1 and 2) showed significant inhibitory activity, with IC(50) values of 30.0 and 6.04 microM, respectively, in this membrane binding assay and acted as norepinephrine reuptake inhibitors through blocking norepinephrine transport.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antidepressive Agents* / chemistry
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Antidepressive Agents* / isolation & purification
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Antidepressive Agents* / pharmacology
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Cocaine / analogs & derivatives
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Cocaine / pharmacology
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Desipramine / pharmacology
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Molecular Structure
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Norepinephrine Plasma Membrane Transport Proteins / metabolism
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Nuclear Magnetic Resonance, Biomolecular
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Oligosaccharides* / chemistry
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Oligosaccharides* / isolation & purification
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Oligosaccharides* / pharmacology
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Plant Roots / chemistry
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Plants, Medicinal / chemistry*
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Polygala / chemistry*
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Taiwan
Substances
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Antidepressive Agents
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Norepinephrine Plasma Membrane Transport Proteins
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Oligosaccharides
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2beta-carbomethoxy-3beta-(4-iodophenyl)tropane
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Cocaine
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Desipramine